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Updated: Jun 27, 2026
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Published on: June 14, 2013
[Detection of the SRY gene by primed in situ labeling]
Yong Nie1, Xianping Ding, Li Deng
1Institute of Medical Genetics, Sichuan University School of Life Science, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, Sichuan 610064, P. R. China.
Objective:
To establish a primed in situ labeling (PRINS) technique which can be more effective in detection of single copy gene.
Methods:
On the basis of traditional PRINS, new reagents and procedures, such as TaqStart antibody, four primers of the sex determining region Y (SRY) gene and TSA(TM) Biotin System were included in detection of the SRY gene. Meanwhile, fluorescence in situ hybridization(FISH) to detect the SRY gene was used as control.
Results:
Fifty metaphases were scored. PRINS labeling showed signals for the SRY on the Y chromosome at band Yp11.3 in all metaphases. These signals were as distinct as that from results of FISH.
Conclusion:
This improved method is ideal for rapidly localizing single copy genes and small DNA segments. And PRINS is a cost- and time-effective alternative to FISH.
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