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In Vitro Evaluation of The Effects Of Er,Cr:YSGG and Diode Lasers Used on Titanium Cylinder
Published on: June 6, 2025
Cellular responses on anodized titanium discs after laser irradiation.
Joo-Hee Lee1, Seong-Joo Heo, Jai-Young Koak
1Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan, Seoul, Korea.
Lasers in Surgery and Medicine
|December 10, 2008
Summary
Laser irradiation of titanium surfaces with CO(2) (carbon dioxide) and Er,Cr:YSGG (erbium chromium-doped yttrium scandium gallium garnet) lasers positively affects osteoblast-like cell proliferation and differentiation. This suggests potential benefits for dental implantology.
Area of Science:
- Biomaterials Science
- Cell Biology
- Laser Dentistry
Background:
- Limited literature exists on laser irradiation's biological effects on titanium surfaces.
- Titanium is widely used in dental applications.
- Understanding laser-surface interactions is crucial for optimizing dental procedures.
Purpose of the Study:
- To investigate osteoblast-like cell responses to laser-irradiated anodized titanium discs.
- To evaluate the effects of CO(2) and Er,Cr:YSGG lasers on cellular proliferation and differentiation.
- To compare different laser irradiation parameters on titanium surfaces.
Main Methods:
- Osteoblast-like HOS cells cultured on four groups of anodized titanium discs: control, CO(2) laser, Er,Cr:YSGG laser (150 J/cm(2)), and Er,Cr:YSGG laser (300 J/cm(2)).
- MTS assay for cell proliferation and alkaline phosphatase (ALP) activity test for differentiation.
- Data analyzed using three-way ANOVA and post hoc tests.
Main Results:
- All substrates supported cell proliferation, with the highest observed in the 300 J/cm(2) Er,Cr:YSGG laser group.
- Laser-irradiated groups (CO(2) and Er,Cr:YSGG) showed significantly higher ALP activity than the control, except for the 150 J/cm(2) Er,Cr:YSGG group.
- Cellular proliferation and differentiation markers increased over time (1 to 3 days) across all groups.
Conclusions:
- CO(2) and Er,Cr:YSGG laser irradiation can positively influence osteoblast proliferation and differentiation on titanium surfaces.
- Specific laser parameters, like higher Er,Cr:YSGG energy density, may enhance these effects.
- Findings suggest potential for laser treatment to improve osseointegration in dental implants.