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Related Concept Videos

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

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Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

A correlative light and electron microscopy method based on laser micropatterning and etching.

Julien Colombelli1, Carolina Tängemo, Uta Haselman

  • 1Cell Biology and Cell Biophysics Unit, EMBL, Heidelberg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2008
PubMed
Summary

This study introduces laser etching as a novel technique for correlative microscopy, enabling precise cell localization for both light and electron microscopy. This method simplifies the workflow for studying cellular structures like the Golgi apparatus.

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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
09:10

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Nanotechnology

Background:

  • Correlative microscopy combines light and electron microscopy for high-resolution imaging.
  • A key challenge is developing compatible labeling techniques for both microscopy types.
  • Existing methods often lack efficiency or ease of use.

Purpose of the Study:

  • To develop a novel correlative light and electron microscopy (CLEM) method.
  • To overcome limitations in sample preparation and localization for CLEM.
  • To apply the new method to study the Golgi apparatus.

Main Methods:

  • Utilizing laser etching to inscribe cell locations onto glass slides.
  • Employing laser-etched ridges for precise sample alignment in microscopy.
  • Integrating light microscopy (fluorescence) with electron microscopy (EM).

Main Results:

  • Demonstrated successful laser inscription for cell identification in light microscopy.
  • Showcased laser-etched inverse ridges for accurate EM sample navigation.
  • Successfully applied the technique to image the Golgi apparatus with high precision.

Conclusions:

  • Laser etching provides a robust and versatile tool for correlative microscopy.
  • This technique simplifies sample handling and improves localization accuracy.
  • Offers a new avenue for high-resolution imaging of subcellular structures.