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[Determination of lobetyolin in root of Codonopsis tangshen from various cultivation areas by high-performance liquid
Dan Song1, Xue-mei Cheng, Long-yun Li
1Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese medicine, Shanghai 201203, China.
Objective:
To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas.
Method:
A Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm.
Result:
Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1).
Conclusion:
The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.
