Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Reaggregate Thymus Cultures
05:47

Reaggregate Thymus Cultures

Published on: August 28, 2008

Reaggregate thymus cultures.

Andrea White1, Eric Jenkinson, Graham Anderson

  • 1MRC Center for Immune Regulation, University of Birmingham. A.white@bham.ac.uk

Journal of Visualized Experiments : Jove
|December 11, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thymus health is a predictor of lifelong well-being and immunotherapy effectiveness.

Nature·2026
Same author

When immunity multitasks: type 2 pathways power thymus regeneration.

Immunology letters·2026
Same author

Claudin 1-mediated positioning of DC1 to mTECs is essential for maintenance of central tolerance.

The Journal of experimental medicine·2026
Same author

Regulatory T cell stability determines the efficiency of bile duct regeneration during cholangitis.

Cell reports·2025
Same author

PD-1 receptor deficiency enhances CD30<sup>+</sup> T<sub>reg</sub> cell function in melanoma.

Nature immunology·2025
Same author

Exploratory Cost-Effectiveness of a Novel Bioprosthetic Valve for Surgical Aortic Valve Replacement in Spain.

PharmacoEconomics - open·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Researchers developed a new method to study T-cell development. By reassembling fetal thymus cells into 3D structures, they can investigate how stromal cells guide immune cell development and self/non-self discrimination.

Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Lymphoid tissue stromal cells form 3D structures crucial for hematopoietic cell development.
  • 2D cultures often fail to replicate the complex functions of 3D lymphoid tissues.
  • Thymic epithelial cells create a framework essential for T-cell development and immune tolerance.

Purpose of the Study:

  • To investigate the functional roles of specific stromal compartments within the thymus.
  • To understand how the thymus facilitates self/non-self discrimination during T-cell development.
  • To develop a method for studying T-cell development under defined cellular conditions.

Main Methods:

  • Enzymatic disaggregation of fetal thymus lobes into heterogeneous cell mixtures.

More Related Videos

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
08:02

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Published on: June 27, 2016

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

Related Experiment Videos

Last Updated: Jun 27, 2026

Reaggregate Thymus Cultures
05:47

Reaggregate Thymus Cultures

Published on: August 28, 2008

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel
08:02

Promoting 3-D Aggregation of FACS Purified Thymic Epithelial Cells with EAK 16-II/EAKIIH6 Self-assembling Hydrogel

Published on: June 27, 2016

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

  • Separation of disaggregated cells into individual cellular components.
  • In vitro re-association of specific cell types into defined 3D reaggregate structures.

    • Main Results:

    • A technique was established to create functional 3D reaggregate structures from fetal thymus cells.
    • This method allows for the investigation of T-cell development under controlled cellular conditions.
    • The plasticity of fetal tissues was exploited for in vitro re-assembly.

    Conclusions:

    • The developed technique enables precise investigation of stromal cell function in T-cell development.
    • Understanding 3D stromal organization is key to deciphering thymic immune education.
    • This approach offers new possibilities for studying immune system development and function.