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Related Experiment Video

Updated: Jun 27, 2026

Generation of Stable Transgenic C. elegans Using Microinjection
12:09

Generation of Stable Transgenic C. elegans Using Microinjection

Published on: August 15, 2008

Generation of stable transgenic C. elegans using microinjection.

Laura A Berkowitz1, Adam L Knight, Guy A Caldwell

  • 1Department of Biological Sciences, University of Alabama, USA. laberkowitz@bama.ua.edu

Journal of Visualized Experiments : Jove
|December 11, 2008
PubMed
Summary
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Generating transgenic Caenorhabditis elegans (C. elegans) involves microinjecting DNA plasmids into the gonad. This method creates stable, extrachromosomal DNA, enabling gene expression studies in various tissues.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Transgenic organisms are crucial for studying gene function.
  • Caenorhabditis elegans is a widely used model organism in biological research.
  • Efficient methods for generating transgenic C. elegans are essential for genetic studies.

Purpose of the Study:

  • To describe the microinjection technique for creating transgenic Caenorhabditis elegans.
  • To explain the process of extrachromosomal concatemer formation and inheritance.
  • To detail the selection and maintenance of stable transgenic C. elegans lines.

Main Methods:

  • Microinjection of DNA plasmid solution into the C. elegans gonad.
  • Co-injection of a gene of interest with a phenotypic marker (e.g., rol-6, GFP).

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Last Updated: Jun 27, 2026

Generation of Stable Transgenic C. elegans Using Microinjection
12:09

Generation of Stable Transgenic C. elegans Using Microinjection

Published on: August 15, 2008

Generation of Transgenic C. elegans by Biolistic Transformation
10:15

Generation of Transgenic C. elegans by Biolistic Transformation

Published on: August 23, 2010

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome
06:33

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Published on: December 9, 2013

  • Selection of transgenic animals using a dissecting microscope.
  • Expression of transgenes from native or tissue-specific promoters.

    • Main Results:

    • Extrachromosomal DNA concatamers are formed and stably inherited.
    • Transgenic C. elegans lines can be generated efficiently.
    • Gene expression is driven in most tissues, excluding the germline and early embryo.

    Conclusions:

    • Microinjection is a versatile technique for generating transgenic C. elegans.
    • This method facilitates diverse experimental applications, including gene expression and localization studies.
    • Stable transgenic C. elegans lines are valuable tools for biological research.