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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jun 27, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

Staining proteins in gels.

Sean Gallagher1, Deb Chakavarti

  • 1Keck Graduate Institute of Applied Life Sciences, UVP, LLC, USA.

Journal of Visualized Experiments : Jove
|December 11, 2008
PubMed
Summary
This summary is machine-generated.

This study details four protein staining methods after gel electrophoresis. Coomassie blue offers speed, while silver and fluorescent stains provide enhanced sensitivity for detecting low-abundance proteins.

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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
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Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Related Experiment Videos

Last Updated: Jun 27, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 8, 2008

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
07:47

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Published on: August 14, 2009

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
06:24

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Published on: April 21, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein detection in gels is crucial after electrophoretic separation.
  • Various staining techniques exist, each with unique sensitivity and speed.
  • Choosing the right method impacts the ability to visualize and quantify proteins.

Purpose of the Study:

  • To describe protocols for four popular protein gel staining methods.
  • To compare the advantages and disadvantages of each staining technique.
  • To provide practical guidance for researchers in protein analysis.

Main Methods:

  • Detailed protocols for Coomassie blue staining.
  • Procedures for highly sensitive silver staining.
  • Methods for fluorescent staining, including SYPRO Orange and SYPRO Ruby.

Main Results:

  • Coomassie blue staining is rapid and straightforward.
  • Silver staining offers superior sensitivity for detecting low protein amounts.
  • Fluorescent staining provides high sensitivity, comparable to or exceeding silver staining.

Conclusions:

  • The choice of protein staining method depends on sensitivity requirements and time constraints.
  • Coomassie blue is suitable for abundant proteins, while silver and fluorescent stains are ideal for trace amounts.
  • This unit provides a comprehensive guide to selecting and implementing effective protein gel staining protocols.