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Brain Imaging

Brain Imaging

Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic Stimulation (TMS).

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Related Experiment Video

Updated: Jun 27, 2026

A Craniotomy Surgery Procedure for Chronic Brain Imaging

A Craniotomy Surgery Procedure for Chronic Brain Imaging

Published on: February 15, 2008

A craniotomy surgery procedure for chronic brain imaging.

Ricardo Mostany¹, Carlos Portera-Cailliau

¹Department of Neurology, University of California, Los Angeles, USA. mostany@ucla.edu

Journal of Visualized Experiments : Jove

|December 11, 2008

Summary

This summary is machine-generated.

This video demonstrates a chronic in vivo two-photon microscopy preparation for studying live brain function. This advanced imaging method allows long-term observation of cortical dynamics and neuronal activity in intact brains.

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Last Updated: Jun 27, 2026

A Craniotomy Surgery Procedure for Chronic Brain Imaging

A Craniotomy Surgery Procedure for Chronic Brain Imaging

Published on: February 15, 2008

A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

A Large Lateral Craniotomy Procedure for Mesoscale Wide-field Optical Imaging of Brain Activity

Published on: May 7, 2017

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

Craniotomy Procedure for Visualizing Neuronal Activities in Hippocampus of Behaving Mice

Published on: July 24, 2021

Area of Science:

Neuroscience
Microscopy
Brain Imaging

Background:

Advanced imaging techniques are crucial for understanding brain function.
Two-photon laser scanning microscopy (TPLSM) enables in vivo studies of the live, intact brain.
TPLSM allows chronic observation of the same cortical area over extended periods.

Purpose of the Study:

To demonstrate a preparation method for chronic in vivo brain imaging using TPLSM.
To highlight the utility of TPLSM for long-term neurological studies.

Main Methods:

Detailed surgical preparation for chronic in vivo imaging.
Creation of long-lasting, glass-covered cranial windows.
Use of sterile conditions and careful surgical technique to minimize damage.

Main Results:

Successful chronic in vivo imaging of the brain is achievable.
The preparation allows for longitudinal studies of neocortical structure and dynamics.
Deep imaging of the neocortex is possible with optimized procedures.

Conclusions:

The presented preparation method facilitates advanced in vivo neuroscience research.
This technique supports various imaging applications, including structural dynamics, neuronal activity, and blood flow.
Careful surgical execution is key for successful, long-term cranial window implantation.