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Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Jun 27, 2026

A Chromatin Assay for Human Brain Tissue

A Chromatin Assay for Human Brain Tissue

Published on: March 21, 2008

A chromatin assay for human brain tissue.

Anouch Matevossian¹, Schahram Akbarian

¹Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, USA.

Journal of Visualized Experiments : Jove

|December 11, 2008

Summary

This study introduces a rapid native chromatin immunoprecipitation (NChIP) method for analyzing epigenetic modifications in postmortem human brain tissue. This technique allows for the investigation of gene expression in neuropsychiatric disorders.

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A Chromatin Assay for Human Brain Tissue

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Published on: January 29, 2018

Area of Science:

Neuroscience
Genetics
Molecular Biology

Background:

Chronic neuropsychiatric illnesses are linked to genetic and environmental factors, potentially involving epigenetic alterations.
Traditional postmortem brain studies are limited by autolysis and tissue integrity, impacting chromatin structure analysis.
Nucleosomal organization is preserved in postmortem brain, enabling epigenetic studies.

Purpose of the Study:

To present a simplified native chromatin immunoprecipitation (NChIP) protocol for frozen human brain specimens.
To enable the study of epigenetic mechanisms in normal and diseased human brain.
To overcome limitations of traditional postmortem brain research.

Main Methods:

Developed a simplified native chromatin immunoprecipitation (NChIP) protocol.
Utilized micrococcal nuclease digestion of brain homogenates.
Combined NChIP with quantitative polymerase chain reaction (qPCR) for analysis.

Main Results:

The NChIP protocol is effective for frozen human brain specimens.
The entire process, from digestion to qPCR, can be completed within three days.
The method preserves essential chromatin structures for analysis.

Conclusions:

The presented NChIP protocol is a valuable tool for studying epigenetic modifications in postmortem human brain.
This methodology can elucidate epigenetic mechanisms underlying gene expression in neuropsychiatric disorders.
The rapid three-day protocol facilitates research on brain diseases.