Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A guide to the ecological limitations of phage therapy of bacterial biofilms: Is what's best for the phage best for the physician?

The FEBS journal·2026

Same author

From hype to hope: reanimating phage therapy through evidence-based multidisciplinarity.

Nature communications·2026

Same author

Bacteriophage titering by optical density means: KOTE assays.

Open life sciences·2026

Same author

Editorial: Emerging trends in phage therapeutics to overcome antibiotic resistance.

Frontiers in antibiotics·2026

Same author

Broth Optical Density-Based Assessment for Phage Therapy: Turbidity Reduction, Antibacterial Virulence, and Time-Kill.

Viruses·2026

Same author

Optical Density-Based Methods in Phage Biology: Titering, Lysis Timing, Host Range, and Phage-Resistance Evolution.

Viruses·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

Published on: June 30, 2023

Practical methods for determining phage growth parameters.

Paul Hyman¹, Stephen T Abedon

¹MedCentral College of Nursing, Mansfield, OH, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 11, 2008

Summary

This summary is machine-generated.

This study details protocols for measuring bacteriophage growth parameters, including adsorption rate, latent period, eclipse period, and burst size, crucial for understanding phage population dynamics.

More Related Videos

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

Published on: June 30, 2023

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

Published on: June 11, 2015

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

Area of Science:

Microbiology
Virology
Molecular Biology

Background:

Bacteriophage growth involves sequential steps: adsorption, nucleic acid uptake, eclipse, maturation, and release.
The latent period encompasses eclipse and maturation, ending with progeny virion release for lytic phages.
Phage adsorption is a complex process involving virion release, extracellular search, collision, and attachment.

Purpose of the Study:

To present protocols for determining key bacteriophage growth parameters.
To elucidate the kinetics of bacteriophage replication cycles.
To provide methods for quantifying phage population growth rates.

Main Methods:

Detailed protocols for measuring phage adsorption rate.
Methods for determining the latent period and eclipse period of phage infection.
Procedures for quantifying phage burst size per infection.

Main Results:

Established methodologies for precise measurement of phage growth parameters.
Quantified the duration of critical stages in the bacteriophage life cycle.
Provided a framework for analyzing phage population dynamics.

Conclusions:

Accurate determination of phage growth parameters is essential for understanding phage biology.
These protocols enable quantitative analysis of bacteriophage replication and population dynamics.
The presented methods are vital for research in phage therapy and microbial ecology.