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Related Experiment Video

Updated: Jun 27, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Baculovirus-transduced mouse amniotic fluid-derived stem cells maintain differentiation potential.

Zheng-Shan Liu1, Yong-Feng Xu, Shan-Wei Feng

  • 1Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

Annals of Hematology
|December 11, 2008
PubMed
Summary

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Baculovirus efficiently transduces mouse amniotic fluid-derived stem cells (mAFSs), a promising gene therapy vector. Genetically modified mAFSs retain their differentiation potential for regenerative medicine applications.

Area of Science:

  • Regenerative Medicine
  • Stem Cell Biology
  • Gene Therapy

Background:

  • Amniotic fluid-derived stem cells show potential in regenerative medicine.
  • Genetic modification may enhance stem cell regenerative capabilities.

Purpose of the Study:

  • To evaluate baculovirus as a novel gene vector for mouse amniotic fluid-derived stem cells (mAFSs).
  • To assess the efficiency and safety of baculovirus transduction in mAFSs.
  • To determine if transduced mAFSs retain their differentiation potential.

Main Methods:

  • Isolation and in vitro culture of mouse amniotic fluid-derived stem cells.
  • Phenotypic analysis (CD29, Sca-1, CD34, CD45, CD11b) and differentiation potential assessment.
  • Transduction of mAFSs using baculovirus vector.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Last Updated: Jun 27, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

  • Induction of differentiation in transduced mAFSs into adipogenic, osteogenic, myogenic, and neurogenic lineages.

    • Main Results:

    • Successfully isolated and cultured mAFSs expressing CD29 and Sca-1, negative for CD34, CD45, and CD11b.
    • mAFSs demonstrated multipotent differentiation into adipocytes, osteocytes, myocytes, and neurocytes in vitro.
    • Baculovirus efficiently transduced mAFSs without compromising their differentiation capacity.

    Conclusions:

    • Baculovirus serves as an effective and safe gene vector for mAFSs.
    • Genetically modified mAFSs retain their differentiation potential, enhancing their suitability for regenerative medicine.
    • Baculovirus-mediated genetic modification offers a promising approach for advancing mAFS-based therapies.