Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pinching-off of Coated Vesicles

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

Clathrin Coated Vesicles

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...

COP Coated Vesicles

COP Coated Vesicles

Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of different...

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dynamic Interplay in Tumor Ecosystems: Communication between Hepatoma Cells and Fibroblasts.

International journal of molecular sciences·2023

Same author

BMP-induced non-canonical signaling is upregulated during autophagy-mediated regeneration in inflamed mesothelial cells.

Scientific reports·2023

Same author

Inflammation in Focus: The Beginning and the End.

Pathology oncology research : POR·2022

Same author

Pinealocytes can not transport neurotropic viruses. Pinealo-to-retinal connection in prepubertal rats originates from pineal neurons: Light and electron microscopic immunohistochemical studies.

Neuroscience letters·2020

Same author

Cellular and molecular events of inflammation induced transdifferentiation (EMT) and regeneration (MET) in mesenteric mesothelial cells.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2020

Same author

Preconditioning with cell-free DNA prevents DSS-colitis by promoting cell protective autophagy.

Journal of investigative medicine : the official publication of the American Federation for Clinical Research·2020

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

Ocadaic acid retains caveolae in multicaveolar clusters.

Anna L Kiss¹, Erzsébet Botos

¹Department of Human Morphology and Developmental Biology, Semmelweis University, 1094 Budapest, Tuzoltó u. 58, Hungary. KissA@ana2.sote.hu

Pathology Oncology Research : POR

|December 11, 2008

Summary

Caveola-mediated endocytosis, a selective cellular uptake process, was investigated. Using an inhibitor, researchers found that caveolar clusters remain connected to the cell surface, accumulating rather than trafficking to late endosomes.

More Related Videos

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

Measuring Fast Calcium Fluxes in Cardiomyocytes

Measuring Fast Calcium Fluxes in Cardiomyocytes

Published on: November 29, 2011

Related Experiment Videos

Last Updated: Jun 27, 2026

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Published on: July 27, 2022

Measuring Fast Calcium Fluxes in Cardiomyocytes

Measuring Fast Calcium Fluxes in Cardiomyocytes

Published on: November 29, 2011

Area of Science:

Cell Biology
Molecular Biology
Endocytosis Research

Background:

Caveola-mediated endocytosis is a distinct cellular uptake pathway.
The nature of caveosomes and their interaction with other endocytic compartments remain unclear.
Regulation of caveola internalization and trafficking is not fully understood.

Purpose of the Study:

To investigate the fate of caveolae after internalization.
To determine if caveolar clusters are independent organelles or remain connected to the cell surface.
To explore the role of serine/threonine phosphatases (PP1 and PP2A) in regulating caveola trafficking.

Main Methods:

Stimulation of caveola internalization using okadaic acid (OA), a PP1 and PP2A inhibitor.
Utilizing ruthenium red (Ru red) as an electron-dense surface marker.
Performing double labeling experiments on ultrathin frozen sections for electron microscopy.

Main Results:

Okadaic acid treatment significantly increased the number of caveolar clusters.
Most OA-induced caveolar clusters were positive for ruthenium red, indicating cell surface connection.
Caveolae accumulated in large multicaveolar clusters and were not transported to late endosomes.

Conclusions:

Caveolar clusters, under OA treatment, remain connected to the cell surface.
Caveolae do not traffic to late endosomes but accumulate in distinct clusters.
Protein phosphatase 2A (PP2A) may play a crucial role in regulating endocytic compartment fusion and microtubule trafficking.