Related Experiment Video
Updated: Jun 27, 2026

Morphometric Analyses of Retinal Sections
Published on: February 19, 2012
Representing the retinal line spread shape with mathematical functions
Yi-Rong Yang1, Justin Wanek, Mahnaz Shahidi
1Department of Bioengineering, University of Illinois at Chicago, IL 60607, USA. yyang19@uic.edu
Objective:
To report a mathematical function that characterizes the double-pass line spread function (LSF) of the human eye. Determining analytical functions that represent the double-pass LSF is important because it allows modeling the optical performance of the eye.
Methods:
Optical section retinal images, generated in normal human eyes using a modified slit-lamp biomicroscope, were analyzed to derive the double-pass LSF by plotting the intensity distribution of laser light reflected/ scattered from the vitreoretinal interface. Three mathematical functions (Lorentzian, Gaussian, exponential) were fitted to the double-pass LSF and the root mean square error (RMSE) was calculated to provide a measure of the goodness of fit.
Results:
The Lorentzian function provided the best representation of the double-pass LSF of normal human eyes. The full width at half maximum (FWHM) of the Lorentzian fitted curve was positively correlated with age, indicating that the double-pass LSF broadens with age. Furthermore, the goodness of fit of the Lorentzian function was significantly better in younger subjects as compared with older subjects, suggesting that the fitted function to the double-pass LSF may vary according to age.
Conclusion:
The results demonstrate an age-related change in the double-pass LSF width and the goodness of fit of the Lorentzian function.
More Related Videos
08:42Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks
Published on: August 4, 2018
06:16LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
Published on: July 28, 2023
Related Concept Videos
Polar Curves
Lines in Space
The Retina
Anatomy of the Eyeball
Transformations of Functions III
Graphs of Polar Equations