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Updated: Jun 27, 2026
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Published on: March 10, 2017
[The effect of IL-10 on proliferation of primary cultured hepatocytes]
Xian-Ping Lin1, Yue-Hong Huang, Wei-da Zheng
1Department of Gastroenterology, Union Hospital, Fujian Medical University, Fuzhou 350001, China. linxiangping001@163.com
Aim:
To investigate the effect of IL-10 on proliferation of rat primary cultured hepatocytes.
Methods:
Rat hepatocytes were isolated from rat liver by in situ digestion of collagenase IV and cryopreserved, resuscitated, cultured in vitro. Reverse-transcription polymerase chain reaction (RT-PCR) was used to characterize the purity of hepatocytes and analyze IL-10/IL10Ralpha mRNA from freshly isolated cells. The primary cultured hepatocytes were divided into 3 groups and treated with nothing (group N), Insulin (group C), and IL-10 in combination with Insulin (group I), respectively. Nuclear cell cycle analysis, MTT, and Trypan Blue cell count was assayed.
Results:
RT-PCR showed expression of characterization genes in primary hepatocytes group and liver tissue are different. RT-PCR showed an expression of IL-10/IL10Ralpha mRNA in rat primary hepatocytes. Trypan Blue cell count showed an depression of cell quantity in group I at 48h (71.96% contrast to group C, P<0.05). MTT analyse also showed absorbance of group I was declined contrast to group C at 24 h and 48 h (88.41% and 90.24%, P<0.05). Cell cycle analysis via FCM showed a decline at 24h in group I than group C and group N (59.06% and 70.18%, P<0.01).
Conclusion:
The primary hepatocytes we isolated is quite purity. Rat primary hepatocytes express IL-10/IL10Ralpha mRNA. IL-10 has an suppression effect on proliferation of primary cultured hepatocytes.
