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Updated: Jun 27, 2026
Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
Published on: May 6, 2013
[Homing of CFSE-labeled antigen-specific CD4(+)CD25(+) regulatory T cells in islets transplantation]
Mei Zhang1, Shu-Hang Xu, Yu Xu
1Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Aim:
To observe the distribution and proliferation of the antigen-specific CD4(+)CD25(+) regulatory T (Treg ) cells and investigate the potential mechanism of antigen-specific CD4(+)CD25(+) regulatory T cells on the suppression of rejection for allogenetic islet transplantation in vivo.
Methods:
Antigen-specific CD4(+)CD25(+) Treg cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. After labeled by CFSE, 8x10(5) antigen-specific Treg cells were injected via tail vein with islets transplantation. Homing and distribution of antigen-specific CD4(+)CD25(+) Treg cells were investigated by flow cytometric analysis and immunofluorescence.
Results:
In vivo, the mean survival time of recipients with islets and antigen-specific CD4(+)CD25(+) Treg cells were (34.57+/-17.15) days, whereas transplanted islets without Treg treatment survived (10.6+/-1.82) days in control mice. The transferred antigen-specific CD4(+)CD25(+) Treg cells were mainly resident in pancreatic node and mesenteric node.
Conclusion:
Allogeneic antigen-specific CD4(+)CD25(+) Treg cells prolong islet graft survival, and draining lymphoid tissue play a key role in the immune response.
