Related Experiment Video
Updated: Jun 27, 2026
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
[Effect of Fe2+ concentration on kinetics of biohydrogen production]
1Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084, China. oio68987045@yahoo.com.cn
Abstract:
The effect of Fe2+ concentration ranging from 0 to 1500 mg/L on the kinetics of fermentative hydrogen production by mixed microbial culture was investigated. The results showed that, at 35 degrees C and initial pH 7.0, using glucose as substrate, hydrogen production potential and average hydrogen production rate increased with increasing Fe2+ concentration from 0 to 300 mg/L, with the maximum hydrogen production potential of 302.3 mL and maximum average hydrogen production rate of 30.0 mL/h being obtained at Fe2+ concentration of 300 mg/L. Hydrogen yield increased with increasing Fe2+ concentration from 0 to 350 mg/L, with the maximum hydrogen yield of 311.2 mL/g glucose being obtained at Fe2+ concentration of 350 mg/L. Modified Logistic model could describe the progress of cumulative hydrogen production in the batch tests successfully. Modified Han-Levenspiel model could describe the effect of Fe2+ concentrations on average hydrogen production rate successfully.
More Related Videos
Related Concept Videos
Catalysis
Concentration and Rate Law
For example, in a generic reaction aA + bB ⟶ products, where a and b are stoichiometric coefficients, the rate law can be written as:
Bioreactor Controls-II
Introduction to Enzyme Kinetics
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
Measuring Reaction Rates
Ladder Diagrams: Redox Equilibria
Consider the Fe3+/Fe2+ half-reaction, which has a standard-state potential of +0.771 V. At potentials more positive than +0.771 V, Fe3+ predominates, whereas Fe2+...

