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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

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Related Experiment Video

Updated: Jun 27, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
09:07

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

[Study on the validation of application model on embryonic stem test].

Zhou Yu1, Weixing Yan, Lianfeng Zhang

  • 1Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China.

Wei Sheng Yan Jiu = Journal of Hygiene Research
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

This study validates an embryonic stem cell test (EST) model for assessing chemical safety. The EST model accurately differentiates embryo-toxic effects of compounds like Penicillin G, DPH, and 5-FU.

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Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
08:47

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Published on: May 15, 2020

Area of Science:

  • Developmental toxicology
  • Stem cell biology
  • In vitro toxicology

Background:

  • Embryonic stem cell (ESC) differentiation into cardiomyocytes is a key area for developmental toxicity testing.
  • Existing safety evaluation methods require improvement for accuracy and efficiency.
  • A validated model is crucial for reliable prediction of embryonic toxicity.

Purpose of the Study:

  • To validate a newly established Embryonic Stem Cell Test (EST) model.
  • To assess the utility of the EST model in differentiating embryonic toxicity.
  • To enhance the safety evaluation methods for chemical compounds.

Main Methods:

  • Embryonic stem cells were differentiated into cardiomyocytes under varying concentrations of Penicillin G, DPH, and 5-FU.
  • Hanging and suspending culture conditions were employed.
  • Cytotoxicity assays were performed to determine embryonic toxicity characteristics.

Main Results:

  • The ID50 (D3) concentrations for Penicillin G, DPH, and 5-FU were determined as 1099, 47.4, and 0.023 microg/ml, respectively.
  • Compounds were successfully discriminated into none-embryotoxicity (Penicillin G), weak-embryotoxicity (DPH), and strong-embryotoxicity (5-FU) categories.
  • The EST model demonstrated high accuracy in classifying tested substances.

Conclusions:

  • The established EST model effectively differentiates between compounds with varying degrees of embryonic toxicity.
  • The validation confirms the high reliability of this EST model for safety assessments.
  • This validated model offers a promising advancement in predicting chemical safety during embryonic development.