Related Experiment Video
Updated: Jun 27, 2026
Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
Published on: October 23, 2011
[Rapid detection method of three types of bacterial pathogens in aquatic products established by multiplex PCR]
Xiaojuan Yang1, Qingping Wu, Jumei Zhang
1Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China. xuyang80612@mail.china.com
Objective:
To develop a rapid multiplex PCR (m-PCR) assay for simultaneously detection of three foodborne pathogens in aquatic products.
Methods:
The invasion protein gene (invA) of Salmonella spp., toxR gene (toxR) of Vibrio parahaemolyticus and invasion-associated protein p60 gene (iap) of Listeria monocytogenes were used as the gene targets.
Results:
The multiplex PCR assay could be specific and rapid, and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37 degrees C for 10 h.
Conclusion:
The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.
Related Concept Videos
01:25Rapid Identification of Pathogens
01:28Methods of Classification and Identification