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Updated: Jun 27, 2026
Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
[Study of rapid method on enterotoxigenic Escherichia coli detected by real-time fluoresence quantitative PCR]
Juan Dai1, Yufeng Li, Xiao Yang
1Lab Medicine Department, Chengdu Medical College, Chengdu 610083, China. daijuan_2004_2007@126.com
Objective:
To develop a real-time PCR for detecting enterotoxigenic Escherichia coli (ETEC) based on TaqMan technology.
Methods:
Primers and probes were designed in the coding region of heat-stable enterotoxin, heat-labile enterotoxin of ETEC. ETEC were detected by real-time fluoresence quantitative PCR, making use of the exterior standard curve which was described by several different concentration. The speciality, sensitivity, accuracy, repetition, and stability of real-time fluoresence quantitative PCR system were evaluated.
Results:
Primers and TaqMan probes were suited to the real-time fluoresence quantitative PCR. The assay showed that the method could be rapid, special, sensitive and stabile. The real-time PCR system could detect ETEC between 10(0)-10(7) DNA copies/reaction. The assay should be finished in two hours.
Conclusion:
It was suggested that real-time fluoresence quantitative PCR based on TaqMan probe could be a rapid, sensitive and special method. It is significant that the excellent method could control diarrhoea caused by ETEC.
