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Salt tolerance associations with RAPD markers in Hordeum vulgare L. and H. spontaneum C. Koch
1Department of Crop Production and Plant Breeding, College of Agriculture, Shiraz University, Shiraz, Islamic Republic of Iran.
Abstract:
Randomly Amplified Polymorphic DNAs (RAPDs) were used to search for markers associated with salt tolerance in barley. Initial screens involved growing 63 cultivated and wild barley genotypes in saline conditions and testing for shoot sodium content along with other physiological traits. From these tests 5 tolerant and 5 non-tolerant genotypes were selected. DNA from the tolerant and non-tolerant genotypes were formed into two contrasting bulks and interrogated using 30 different 10-mer RAPD primers. One primer (P15) produced a 5100 bp band found only in non-tolerant genotypes and additionally produced a 1300 bp product found only in the tolerant group. Primer P10 produced a band specific to tolerant bulk and P22 produced a band specific to the non-tolerant group.
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