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Updated: Jun 27, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Effect of nitric oxide on ram sperm motility in vitro
Hossein Hassanpour1, Pejman Mirshokrai, Abolfazl Shirazi
1Department of Basic Sciences, College of Veterinary Medicine, Sharekord University, Sharekord, Iran.
Abstract:
The aim of this study is to investigate the effects of NO on sperm motility of ram. After incubation of normozoospermic samples for 160 min in the presence of L-arginine (NOS substrate), SNP (NO donor), L-NAME (non-selective nitric oxide inhibitor) and packed erythrocytes (NO scavenger), sperm motility assessed in four grades (A, B, C, D). In this study, L-arginine and SNP non-significantly increased progressive motility at low concentration and significantly decreased progressive motility at high concentration. L-NAME and packed erythrocytes dose-dependently decreased progressive sperm motility. It is concluded that sperm motility of ram physiologically depend on nitric oxide action although excess generation of nitric oxide by sperm or exogenous NO could not increase sperm motility and it seems that excess NO provides toxic condition to decrease sperm motility.
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