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Updated: Jun 27, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
Published on: February 5, 2019
Translation initiation modeling and mutational analysis based on the 3(')-end of the Escherichia coli 16S rRNA
Zaher Dawy1, Faruck Morcos, Johanna Weindl
1Department of Electrical and Computer Engineering, American University of Beirut, Riad El Solh, Beirut, Lebanon. zaher.dawy@aub.edu.lb
Abstract:
A model for the process of translation in gene expression is proposed. The model is based on the assumption that the ribosome decodes the mRNA sequences using consecutive subsequences of the 3(')-end of its 16S rRNA subunit. The biological consistency of the model is validated by successful detection of the Shine-Dalgarno signal and the start codon. Furthermore, implications on the role of the 3(')-end in the complete process of prokaryotic translation are presented and discussed. Interestingly, the results obtained support the possibility of an involvement of this part of the ribosome in the process of translation termination. Subsequently, results obtained via the proposed model are compared with published experimental results for different mutations of the last 13 bases of the 16S rRNA molecule. Agreement between predictions and experimental results validate the biological relevance of the proposed model. By means of simulated nucleotide mutations, a global analysis of this part of the ribosome in the process of translation is conducted.
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