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Published on: February 16, 2022
Sensitive determination of phenothiazines in pharmaceutical preparation and biological fluid by flow injection
Yinhuan Li1, Weifen Niu, Jiuru Lu
1School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China; School of Science, Xi'an Jiaotong University, Xi'an 710049, China.
Abstract:
A flow injection chemiluminescence method was described for the determination of four phenothiazine drugs, namely, chlorpromazine hydrochloride, perphenazine hydrochloride, fluphenazine hydrochloride and thioridazine hydrochloride. Strong Chemiluminescence (CL) signal was produced when above-mentioned drug was injected into the mixed stream of luminol with KMnO(4). The linear ranges of the method were 0.0020-1.0mug/mL chlorpromazine hydrochloride, 0.0040-3.0mug/mL perphenazine hydrochloride, 0.0020-5.0mug/mL fluphenazine hydrochloride and 0.0050-1.0mug/mL thioridazine hydrochloride. The detection limits were 0.4ng/mL chlorpromazine hydrochloride, 0.7ng/mL perphenazine hydrochloride, 2ng/mL fluphenazine hydrochloride and 0.7ng/mL thioridazine hydrochloride. The proposed method was applied to the determination of chlorpromazine hydrochloride in injections and in mental patient's urine samples and the satisfactory results were achieved. The possible CL reaction mechanism was also discussed briefly.
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