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Updated: Jun 27, 2026

NiO Nanoflowers for Non-Enzymatic Amperometric Detection of Glucose
Published on: December 30, 2025
Flow-injection analysis of glucose without enzyme based on electrocatalytic oxidation of glucose at a nickel
Changzhi Zhao1, Changli Shao, Minghua Li
1College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Abstract:
This paper reports a flow-injection analysis (FIA) of glucose not using enzyme based on the electrocatalytic oxidation of glucose at a nickel electrode. The electrocatalytic mechanism and quantificational method of glucose have been investigated. The current intensity of the electrocatalytic oxidation to glucose at the potential of 550mV is proportional to the concentration of glucose over the range of 0.10-2.50mmoll(-1), with a 0.04mmoll(-1) detection limit (S/N=3) and a correlation coefficient of 0.9991. The relative standard deviation (R.S.D.) is less than 4.3% (n=5) for the determination of practical serum samples. The biologic compounds probably existed in the sample, such as ascorbic acid, uric acid, dopamine and epinephrine, do not disturb the determination of glucose. The result is satisfactory for the determination of glucose in human serum sample as comparison to that from the routine hexokinase method.
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