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Indirect thermal lens detection for capillary electrophoresis
D A Nedosekin1, S N Bendrysheva, W Faubel
1Analytical Chemistry Division, Chemistry Department, M.V. Lomonosov Moscow State University, Vorob'evy Hills d. 1 Str. 3, 119992 Moscow, Russia.
Talanta
|December 17, 2008
Summary
This study demonstrates sensitive detection of organic anions using thermal lens detection and capillary electrophoresis. Acetonitrile addition significantly enhanced the detection sensitivity for these analytes.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrophoresis (CE) is a powerful separation technique.
- Thermooptical detection offers high sensitivity for analytes.
- Indirect detection methods are crucial for analytes lacking chromophores.
Purpose of the Study:
- To optimize indirect thermooptical detection for organic anions using capillary electrophoresis.
- To evaluate the impact of probe ion concentration and mobile phase composition on detection sensitivity.
Main Methods:
- Utilized thermal lens detection with a 325.0nm He-Cd laser.
- Employed capillary electrophoretic separation for organic anions.
- Optimized separation electrolyte containing Mordant Yellow 7 as a probe ion and Tris buffer.
- Investigated the effect of adding acetonitrile to the separation electrolyte.
Main Results:
- Achieved limits of detection in the order of 10(-7)M for various sulfonic acids and acetic acid.
- Optimized conditions included 50μM Mordant Yellow 7 and 5mM Tris (pH 9.90).
- A twofold decrease in the limit of detection was observed with 20% acetonitrile in the separation electrolyte.
Conclusions:
- Indirect thermal lens detection is a viable and sensitive method for organic anion analysis in CE.
- The addition of acetonitrile to the separation electrolyte significantly enhances detection sensitivity.
- This method provides a valuable tool for the quantitative analysis of organic anions.
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