Related Experiment Video
Updated: Jun 27, 2026

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
Published on: January 30, 2019
Determination of carbonyl compounds in the atmosphere by DNPH derivatization and LC-ESI-MS/MS detection
Yuguang Chi1, Yanli Feng, Sheng Wen
1State Key Laboratory of Organic Geochemistry, Guangzhou Key Laboratory of Environment Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, PR China.
Abstract:
A method of determination of 32 carbonyl compounds by high performance liquid chromatography (HPLC) and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) after derivatization with 2,4-dinitrophenylhydrazine (DNPH) was developed and successfully applied to the atmosphere sample of a residential area of Liwan District (S1) and a research institute of Tianhe District (S2) in Guangzhou, China. Some operation conditions of ESI-MS/MS in the negative ion mode including selection of parent and daughter ions, declustering potential (DP), entrance potential (EP), collision energy (CE), collision cell exit potential (CXP) and effect of buffer in ESI-MS/MS process were optimized. The regression coefficient of the calibration curves (R(2)), recovery, reproducibility (R.S.D., n=5) and limit of detection (LOD) were in the range of 0.9938-0.9999, 90-104%, 1.7-11% and 0.4-9.4ng/m(3), respectively. Among most of the samples, acetone was the most abundant carbonyl in two sampling sites and formaldehyde, acetaldehyde and butyraldehyde/2-butanone were also abundant carbonyls. In contrast to LC-UV method, the LOD, the separation of some co-eluting compounds and the precision (mainly to higher molecular weight carbonyls) are all improved by LC-ESI-MS/MS.
Related Concept Videos
Gas Chromatography: Types of Detectors-II
NMR Spectroscopy and Mass Spectrometry of Aldehydes and Ketones
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Spectroscopy of Carboxylic Acid Derivatives
In the...
Mass Spectrum: Interpretation
High-Performance Liquid Chromatography: Types of Detectors

