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Amperometric glutamate biosensor based on self-assembling glutamate dehydrogenase and dendrimer-encapsulated platinum
Longhua Tang1, Yihua Zhu, Lihuan Xu
1Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
A novel amperometric biosensor based on self-assembling glutamate dehydrogenase (GLDH) and poly(amidoamine) dendrimer-encapsulated platinum nanoparticles (Pt-PAMAM) onto multiwall carbon nanotubes (CNTs) has been developed for the determination of glutamate. The formation of the self-assembled (GLDH/Pt-PAMAM)(n)/CNTs construction was investigated by zeta-potential and high resolution transmission electron microscopy (HRTEM). The results indicated the uniform growth of the layer-by-layer nanostructures onto carboxyl-functionalized CNTs. The electrocatalytic property of the (GLDH/Pt-PAMAM)(n)/CNTs modified electrode to glutamate in presence of NAD(+) (beta-nicotinamide adenine dinucleotide, 0.1mM) was investigated at a low overpotential 0.2V by electrochemical measurements. The results showed it had series of attractive characteristics, such as a large determination range (0.2-250muM), a short response time (within 3s), a high sensitivity (433muA/mM(-1)cm(2)) and good stability (85% remains after 4 weeks).
