Related Experiment Video
Updated: Jun 27, 2026

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy
Published on: May 27, 2022
Evaluation of selenium behavior in thermospray flame furnace atomic absorption spectrometry
Fabiana Rosini1, Clésia C Nascentes, José Y Neira
1Grupo de Análise Instrumental Aplicada, Departamento de Química, Universidade Federal de São Carlos, P.O. Box 676, 13560-970 São Carlos, SP, Brazil.
Abstract:
The behavior of selenium in thermospray flame furnace atomic absorption spectrometry (TS-FF-AAS) was studied and the developed procedure was applied for selenium determination in biological materials after microwave-assisted sample digestion. A sample volume of 600muL was introduced into the hot metallic Ni tube at a flow rate of 0.4mLmin(-1) using water as carrier. The limit of detection obtained for Se was 8.7mugL(-1) (3s(blank)/slope, n=10), which is 95-fold better than that typically obtained using FAAS. The applicability of the TS-FF-AAS procedure was evaluated for selenium determination in biological materials. Certified reference materials of pig kidney (BCR 186) and mussel (GBW 08571) were analyzed and a t-test had not shown any statistically significant difference at a 95% confidence level between determined and certified values for both materials. The procedure was successfully applied for determination of Se in pig kidney and shellfish. It was demonstrated that TS-FF-AAS improved the performance of FAAS (flame atomic absorption spectrometry) for determination of Se.
Related Concept Videos
Atomic Absorption Spectroscopy: Atomization Methods
Atomic Fluorescence Spectroscopy
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Flame Photometry: Overview
Atomic Emission Spectroscopy: Interference
Atomic Emission Spectroscopy: Lab

