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Updated: Jun 27, 2026
Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System
Published on: December 10, 2021
Molecular cloning and characterization of the common marmoset huntingtin gene
Hirohiko Hohjoh1, Hirofumi Akari, Yuko Fujiwara
1Department of Molecular Genetics, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan. hohjohh@ncnp.go.jp
Abstract:
We report here for the first time the isolation and identification of the common marmoset (Callithrix jacchus) huntingtin (Htt) gene, whose ortholog in humans is known to be related to Huntington's disease (HD). A 9396 nucleotide complementary DNA (cDNA) carrying the putative full-length open reading frame of the marmoset Htt gene was identified, and highly conserved nucleotide and amino acid sequences among primates were observed. Based on this data and using tools evaluated for the detection of the marmoset Htt gene, we have demonstrated gene silencing against the expression of endogenous Htt gene in immortalized common marmoset mononuclear cells by means of RNA interference (RNAi). Taken together, the data presented here may assist us in realizing a non-human primate HD model with the common marmoset.
