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Updated: Jun 27, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
[Sequence analysis of the DM domain of three Dmrt genes in loach, Paramisgurnus dabryanus]
Ling-Hua Xu1, Zhen-Jun Yan, Qing-Tao Zeng
1School of Chemical and Material Engineering, Huangshi Institute of Technology, Huangshi Hubei Province, 435003, China. linghua0317@126.com
Abstract:
We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrt1, PdDmrt3, and PdDmrt5. Based on similarities of amino acid sequences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree. It confirmed the identity of the three PCR products. Our results reveal that Dmrt gene is highly conservative in phylogeny.
Insights
We identified three novel Dmrt genes in Paramisgurnus dabryanus, named PdDmrt1, PdDmrt3, and PdDmrt5. Phylogenetic analysis confirmed their identity and revealed the high conservation of Dmrt genes across vertebrate evolution.
Area of Science:
- Molecular Biology
- Genomics
- Evolutionary Biology
Context:
- The DM domain (DM) is a DNA-binding domain found in transcription factors involved in sexual development.
- Understanding the diversity and evolutionary conservation of DM domain genes is crucial for deciphering developmental pathways.
Purpose:
- To identify and characterize novel DM domain-containing genes in the fish species Paramisgurnus dabryanus.
- To investigate the evolutionary relationships of these newly identified genes within the broader vertebrate Dmrt gene family.
Summary:
- Genomic DNA from Paramisgurnus dabryanus was amplified using DM degenerate primers, yielding a ~150 bp band.
- Three sequences, named PdDmrt1, PdDmrt3, and PdDmrt5, were cloned, sequenced, and showed high homology to the DM domain.
- Phylogenetic analysis incorporating Dmrt gene sequences from six vertebrate species confirmed the identity of the PCR products, highlighting the conserved nature of the Dmrt gene family.
Impact:
- This study expands the known repertoire of Dmrt genes in vertebrates.
- The findings provide insights into the evolutionary history and conservation of genes regulating development.
