Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Logistic Regression and Machine Learning Approaches in Predicting Depressive Symptoms: A National-Based Study.

Psychiatry investigation·2025
Same author

Association of Life's Essential 8 with incidence of heart failure modified by depressive symptoms: a prospective cohort study from UK Biobank.

BMC medicine·2025
Same author

Grip strength, genetic predisposition, and Incident Parkinson's disease: a prospective cohort study in the UK Biobank.

NPJ Parkinson's disease·2024
Same author

Wnt/Ca<sup>2+</sup> pathway inhibits neural differentiation of human dental pulp stem cells in vitro.

Journal of dental sciences·2024
Same author

Multimorbidity patterns and the risk of falls among older adults: a community-based study in China.

BMC geriatrics·2024
Same author

YAP promotes the early development of temporomandibular joint bony ankylosis by regulating mesenchymal stem cell function.

Scientific reports·2024

Related Experiment Video

Updated: Jun 27, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

[Expression and function of microRNA in embryonic stem cell].

Shi-Hua Wang1, Chun-Jing Bian, Chun-Hua Zhao

  • 1Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. wshawp@163.com

Yi Chuan = Hereditas
|December 17, 2008
PubMed
Summary

MicroRNAs are small RNAs that regulate gene expression in embryonic stem cells. This review explores how microRNAs control stem cell self-renewal and differentiation through intricate gene networks.

More Related Videos

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
10:19

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Published on: June 24, 2014

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

Related Experiment Videos

Last Updated: Jun 27, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium
10:19

MicroRNA Expression Profiles of Human iPS Cells, Retinal Pigment Epithelium Derived From iPS, and Fetal Retinal Pigment Epithelium

Published on: June 24, 2014

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
03:34

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Epigenetics

Context:

  • Embryonic stem cells (ESCs) possess unique self-renewal and differentiation capabilities essential for development.
  • These processes are intricately controlled by genetic and epigenetic regulatory networks.
  • MicroRNAs (miRNAs), a class of non-coding RNAs, are emerging as critical regulators in ESCs.

Purpose:

  • To review the expression patterns of microRNAs in ESCs.
  • To elucidate the functional roles of specific microRNAs in regulating ESC self-renewal.
  • To examine the involvement of microRNAs in directing ESC differentiation pathways.

Summary:

  • MicroRNAs function as post-transcriptional regulators by binding to target messenger RNAs (mRNAs), leading to mRNA degradation or translational inhibition.
  • Specific microRNAs are uniquely expressed in ESCs, forming complex regulatory networks that target key transcription factors and other genes.
  • This review highlights the critical role of these microRNAs in maintaining pluripotency and guiding differentiation decisions in ESCs.

Impact:

  • Understanding miRNA-mediated regulation in ESCs provides insights into fundamental developmental processes.
  • This knowledge can inform strategies for directed differentiation of ESCs for regenerative medicine applications.
  • Identifying key miRNA regulators may lead to novel therapeutic targets for diseases involving stem cell dysfunction.