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Related Concept Videos

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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Related Experiment Video

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Gene expression and hepatitis C virus infection.

T Asselah1, I Bièche, A Sabbagh

  • 1INSERM, U773, Centre de Recherche Bichat-Beaujon CRB3, Paris, France. tarik.asselah@bjn.aphp.fr

Gut
|December 17, 2008
PubMed
Summary

Hepatitis C virus (HCV) infection causes chronic liver disease. Gene expression profiles can predict disease outcomes and treatment responses in patients with HCV.

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Area of Science:

  • Hepatology
  • Virology
  • Genomics

Background:

  • Hepatitis C virus (HCV) is a global health concern, leading to chronic liver disease, cirrhosis, and hepatocellular carcinoma in millions.
  • Current treatments like pegylated interferon and ribavirin have limited efficacy (55% response rate) and poor tolerability.
  • Host genetic factors significantly influence HCV infection progression and treatment response.

Purpose of the Study:

  • To review the current literature on gene expression in Hepatitis C virus infection.
  • To explore the association between gene expression and fibrosis progression in HCV patients.
  • To analyze gene expression patterns related to treatment response in Hepatitis C virus infection.

Main Methods:

  • Comprehensive literature review of published studies.
  • Analysis of gene expression data related to HCV infection.
  • Correlation of gene expression with fibrosis progression and treatment outcomes.

Main Results:

  • Gene expression profiles offer potential for classifying disease subsets and predicting outcomes.
  • Specific gene expression patterns are linked to the progression of liver fibrosis in HCV patients.
  • Host genetic diversity plays a role in the variability of clinical outcomes and treatment responses.

Conclusions:

  • Gene expression analysis is a promising tool for personalized medicine in Hepatitis C virus infection.
  • Understanding host genetic factors can improve prediction of disease trajectory and treatment success.
  • Further research into gene expression is crucial for developing more effective HCV therapies.