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Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

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Related Experiment Video

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Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

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Published on: November 4, 2019

Human stem cell factor (SCF) is a heparin-binding cytokine.

Satoko Kishimoto¹, Shingo Nakamura, Hidemi Hattori

¹Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan.

Journal of Biochemistry

|December 17, 2008

Summary

Chemically modified heparins bind to human stem cell factor (SCF), requiring specific sulfate groups. Immobilized SCF also stimulates human erythroleukemia cell proliferation.

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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

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Published on: May 17, 2021

Area of Science:

Biochemistry
Cell Biology
Biotechnology

Background:

Heparin and its derivatives are known for diverse biological activities.
Stem cell factor (SCF) is a crucial cytokine involved in hematopoiesis and cell survival.
Understanding the molecular interactions between heparin-based molecules and SCF is important for therapeutic development.

Purpose of the Study:

To investigate the binding affinities of chemically modified heparins to human stem cell factor (SCF).
To elucidate the structural requirements of heparin for SCF binding.
To assess the biological effect of SCF immobilized on microparticles.

Main Methods:

Utilized fragmin/protamine microparticles (F/P MPs) for heparin derivative immobilization.
Employed enzyme-linked immunosorbent assay (ELISA) to quantify SCF binding.
Tested inhibition of SCF binding using varying concentrations of heparin, fragmin, and NaCl.

Main Results:

High concentrations of heparin and fragmin inhibited SCF binding to F/P MPs.
SCF binding was also inhibited by high salt concentrations (≥0.55 M NaCl).
Pre-immobilized SCF significantly promoted the proliferation of a human erythroleukemia cell line.

Conclusions:

A high content of all three sulfate groups in heparin's repeating disaccharide units is essential for SCF interaction.
SCF immobilized on F/P MPs exhibits biological activity, stimulating erythroleukemia cell proliferation.
These findings provide insights into heparin-SCF interactions and potential biotechnological applications.