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Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Stem Cell Niche

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Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

Overview of Hematopoiesis

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Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
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Role of Hematopoietic Growth Factors

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Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
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Related Experiment Video

Updated: Jun 27, 2026

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Published on: September 28, 2022

SHIP is required for a functional hematopoietic stem cell niche.

Amy L Hazen¹, Michelle J Smith, Caroline Desponts

¹Immunology Program, H. Lee Moffitt Comprehensive Cancer Center and Research Institute, Tampa, FL, USA.

|December 17, 2008

Summary

SH2-domain-containing inositol 5'-phosphatase-1 (SHIP) is not intrinsically required for hematopoietic stem cell (HSC) function. SHIP is essential within the bone marrow microenvironment to support HSCs for effective blood cell production.

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Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

Published on: August 1, 2017

Area of Science:

Hematology
Stem Cell Biology
Immunology

Background:

SH2-domain-containing inositol 5'-phosphatase-1 (SHIP) deficiency increases hematopoietic stem cell (HSC) numbers but impairs their function.
This suggests SHIP may be intrinsically required for HSCs.

Purpose of the Study:

To determine if SHIP is an intrinsic requirement for HSC function or if it supports HSCs via the bone marrow (BM) microenvironment.
To investigate the role of SHIP in HSCs and the BM niche.

Main Methods:

Developed a mouse model with SHIP ablated specifically in HSCs within a SHIP-competent BM milieu.
Transplanted these SHIP-deficient HSCs and compared their repopulation capacity to wild-type HSCs and systemically SHIP-deficient HSCs.
Analyzed HSC function and BM niche cell function in SHIP-deficient models.

Main Results:

SHIP-deficient HSCs showed normal long-term repopulation and serial transfer capacity when in a SHIP-competent BM milieu.
HSCs from mice with systemic SHIP ablation exhibited compromised repopulation capacity.
Cells within the BM niche express SHIP, and its absence alters niche cell function.

Conclusions:

SHIP is not intrinsically required for HSC function.
SHIP is crucial for the bone marrow microenvironment to support functional HSCs.
SHIP deficiency impacts BM niche cells, affecting HSC support.