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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

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Related Experiment Video

Updated: Jun 27, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Real-time polymerase chain reaction detection methods.

Douglas F Whitman¹, Sherry A Dunbar

¹Luminex Corporation, 12212 Technology Blvd., Austin, TX 78727, USA. dwhitman@luminexcorp.com

Recent Patents on DNA & Gene Sequences

|December 17, 2008

Summary

This summary is machine-generated.

Recent patents reveal diverse chemistries for real-time Polymerase Chain Reaction (PCR) nucleic acid detection. This review covers DNA-binding agents, molecular beacons, and probes for enhanced amplification analysis.

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Last Updated: Jun 27, 2026

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Published on: November 15, 2017

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Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Real-time Polymerase Chain Reaction (PCR) offers advanced nucleic acid detection over traditional end-point methods.
Patent literature reflects significant innovation in real-time PCR assay chemistries.

Purpose of the Study:

To review recent patents on various chemistries for nucleic acid detection in real-time PCR.
To categorize and highlight the advantages and applications of different real-time assay chemistries.

Main Methods:

Literature review of recent patents related to real-time PCR assay chemistries.
Categorization of chemistries into DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer systems.

Main Results:

Identified and categorized key real-time PCR assay chemistries.
Highlighted specific advantages and applications for each category of chemistry.

Conclusions:

Diverse patented chemistries enhance nucleic acid detection in real-time PCR.
Understanding these chemistries is crucial for advancing molecular diagnostics and research.