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Related Experiment Video

Updated: Jun 27, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Published on: January 17, 2014

Membrane remodelling during vaccinia virus morphogenesis.

Francisco Javier Chichón¹, María Josefa Rodríguez, Cristina Risco

¹Macromolecular Complexes Laboratory, Department of Structure of Macromolecules, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas, Campus Universidad Autónoma, Madrid 28049, Spain.

Biology of the Cell

|December 17, 2008

Summary

This summary is machine-generated.

Vaccinia virus (VACV) membrane maturation involves crescent-shaped structures forming immature viruses, which then rearrange their envelopes to become infectious. This process resembles cellular endomembrane dynamics.

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Last Updated: Jun 27, 2026

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

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Area of Science:

Virology
Cell Biology
Structural Biology

Background:

Vaccinia virus (VACV) is a large, complex virus with over 100 structural proteins.
Viral assembly involves intricate sequential interactions and structural component rearrangements.

Purpose of the Study:

To investigate the three-dimensional membrane remodeling during vaccinia virus envelope maturation.
To elucidate the dynamic processes of VACV morphogenesis.

Main Methods:

Electron tomography of vaccinia virus-infected cell sections.
Three-dimensional reconstruction and analysis of viral structures.

Main Results:

Immature VACV forms from interacting 'crescents' that enclose an ellipsoid volume.
DNA incorporation into immature virus with nucleoid (IVN) causes envelope disorganization and spike loss.
IVN transformation into mature virus involves extreme envelope fragmentation and undulation, with membrane internalization observed.

Conclusions:

VACV membrane maturation shares similarities with the breakdown and reassembly of cellular endomembranes.
The study provides insights into the complex morphogenesis of poxviruses.