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A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry
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Tear sample collection using cellulose acetate absorbent filters.

Marieh Esmaeelpour1, Jun Cai, Patrick Watts

  • 1School of Optometry and Vision Sciences, Cardiff University, Maindy Road, Cardiff CF24 4LU, UK. EsmaeelpourM@cf.ac.uk

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

Cellulose acetate filter rods offer a minimally invasive method for tear collection, yielding higher sample volumes and comparable protein concentrations to traditional glass capillary tubes. This technique is suitable for various sample sizes and tear stimulation conditions.

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Area of Science:

  • Ophthalmology
  • Biomaterials Science

Background:

  • Tear collection is crucial for diagnosing and monitoring ocular surface diseases.
  • Traditional methods like glass capillary tubes have limitations in sample volume and invasiveness.

Purpose of the Study:

  • To evaluate cellulose acetate filter rods (CR) as an alternative tear collection technique.
  • To compare CR with glass capillary tubes (CT) regarding sample volume, protein concentration, and invasiveness.

Main Methods:

  • Cellulose acetate rods (CR) were compared against glass capillary tubes (CT).
  • Experiments assessed collected sample volume, effect of tear stimulation on protein concentrations (total protein, IgA, lactoferrin, lysozyme), and technique invasiveness.
  • Bovine albumin standard was used to compare protein extraction efficiency.

Main Results:

  • No significant differences in total protein, IgA, lactoferrin, or lysozyme concentrations were observed between CR and CT.
  • CR collected significantly higher sample volumes, especially under stimulated conditions.
  • CR reliably demonstrated the dilution effect in stimulated samples, unlike CT.
  • CR extracted smaller sample volumes but higher protein concentrations when using a bovine albumin standard.

Conclusions:

  • Cellulose acetate rods provide a suitable, minimally invasive alternative for tear collection.
  • The technique efficiently absorbs tear samples, accommodating a wide range of volumes.
  • CR is effective for tear analysis, including assessing protein concentrations and dilution effects.