Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative profiling of lifespan-dependent cell-cell communication potential reveals dynamic ligand-receptor network shifts across mouse tissues.

PloS one·2026
Same author

Author Correction: Targeting QKI-7 in vivo restores endothelial cell function in diabetes.

Nature communications·2026
Same author

Epigenetic regulation by HDAC11-driven STAT3 activation promotes pro-inflammatory cytokine production and endothelial dysfunction upon bacterial infection in diabetes.

Cell communication and signaling : CCS·2026
Same author

Diabetes-linked metabolic dysfunction relates with distinct tau phosphorylation patterns, neuroinflammation and cognitive impairment in mouse models of Alzheimer's disease.

Brain, behavior, and immunity·2025
Same author

Impaired Efferocytosis of Pericytes and Vascular Smooth Muscle Cells in Diabetic Retinopathy.

Cells·2025
Same author

Scavenging acrolein with 2-HDP preserves neurovascular integrity in a rat model of diabetic retinal disease.

Diabetologia·2025

Related Experiment Video

Updated: Jun 27, 2026

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
06:54

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Published on: November 12, 2017

Protein expression profiling during chick retinal maturation: a proteomics-based approach.

Sorcha Finnegan1, Joanne L Robson, Mildred Wylie

  • 1Centre for Vision Sciences, Queen's University of Belfast, Institute of Clinical Sciences, Royal Victoria Hospital, Belfast, Northern Ireland. s.finnegan@ucc.ie

Proteome Science
|December 17, 2008
PubMed
Summary

This study used proteomic analysis to identify proteins involved in chick retinal development. Key proteins were identified, offering insights into the molecular mechanisms of retinogenesis and synaptogenesis.

More Related Videos

Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
06:54

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Published on: November 12, 2017

Single-cell Profiling of Developing and Mature Retinal Neurons
10:20

Single-cell Profiling of Developing and Mature Retinal Neurons

Published on: April 19, 2012

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs
06:58

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Proteomics

Background:

  • Retinal neurogenesis and synaptogenesis pathways are not fully understood.
  • Protein expression analysis offers direct insight into developmental processes.

Purpose of the Study:

  • To employ proteomic analysis to study the developing chick retina.
  • To investigate protein expression during embryonic (E) development (days 12-13, 17, 19) and post-hatch (P) (days 1, 33).

Main Methods:

  • Utilized 2D proteomic and mass spectrometric analysis.
  • Analyzed protein expression across multiple developmental time points.

Main Results:

  • Detected an average of 1514 protein spots per gel.
  • Identified 15 modulated or constitutively expressed proteins via mass spectrometry.
  • Identified proteins include tubulins, actin, enolase, creatine kinase, nucleophosmin 1, and beta-synuclein.

Conclusions:

  • This study adds a unique dataset to previous proteomic investigations of retinal development.
  • Identified proteins are likely crucial for normal chick retinal development.
  • Further analysis of dynamic protein populations will enhance understanding of retinogenesis molecular events.