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Related Concept Videos

Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Prokaryotes02:35

Replication in Prokaryotes

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DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Replication in Eukaryotes02:31

Replication in Eukaryotes

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Related Experiment Video

Updated: Jun 27, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

ReplicationDomain: a visualization tool and comparative database for genome-wide replication timing data.

Nodin Weddington1, Alexander Stuy, Ichiro Hiratani

  • 1Department of Biological Sciences, Florida State University, Tallahassee, Florida 32306, USA. weddington@bio.fsu.edu

BMC Bioinformatics
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

ReplicationDomain is a new web tool for analyzing DNA replication timing across the genome. It allows researchers to compare replication profiles and upload their own data for private or public sharing.

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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

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Related Experiment Videos

Last Updated: Jun 27, 2026

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
08:06

Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

Published on: January 19, 2017

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Published on: October 27, 2011

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Eukaryotic DNA replication is organized into large chromosomal domains (0.5-5 megabases in mammals) that replicate synchronously.
  • These domains exhibit a specific, cell-type-stable temporal order during S-phase.
  • Genome-wide replication timing analysis reveals stable patterns within specific cell types.

Purpose of the Study:

  • To develop a web-based database for analyzing and comparing genome-wide replication timing maps.
  • To integrate comparative transcriptional expression data and other genome-wide properties.
  • To provide a user-friendly platform for data visualization and sharing.

Main Methods:

  • Development of the ReplicationDomain web-based database (http://www.replicationdomain.org).
  • Integration of genome-wide replication timing maps (replication profiles) from various cell lines and species.
  • Implementation of an interactive web interface for displaying and downloading data, including microarray, ChIP-Chip, and ChIP-Seq data.
  • Inclusion of a user registration system for data upload, private viewing, and sharing.

Main Results:

  • ReplicationDomain provides a platform for the analysis of genome-wide replication timing maps.
  • The database facilitates comparative analysis with transcriptional expression and other genome-wide data.
  • Users can visualize, download, and upload their own replication timing data sets.
  • A user registration system enables private, shared, or public data availability.

Conclusions:

  • ReplicationDomain is a powerful tool for comparative visualization of replication timing and chromatin features.
  • It offers faster and more convenient browsing of multi-megabase chromosomal segments compared to existing tools.
  • The data upload and sharing functionalities make it a valuable resource for the scientific community, supporting data sharing requirements.