Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

TRAIL and Taurolidine induce apoptosis and decrease proliferation in human fibrosarcoma.

Adrien Daigeler1, Christina Brenzel, Daniel Bulut

  • 1Department of Plastic Surgery, Burn Center, Hand Center, Sarcoma Reference Center, BG-University Hospital Bergmannsheil, Bürkle-de-la-Camp-Platz 1, 44789 Bochum, Germany. adrien.daigeler@rub.de

Journal of Experimental & Clinical Cancer Research : CR
|December 17, 2008
PubMed
Summary

Related Concept Videos

Drugs that Stabilize Microtubules01:15

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Millesi's Classification of Nerve Fibrosis Remains Underutilized in Modern Peripheral Nerve Research.

Muscle & nerve·2026
Same author

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2026
Same author

Predicting Burn Injury Consultations: The Influence of Temporal Variables and Weather Conditions.

Journal of burn care & research : official publication of the American Burn Association·2026
Same author

Maturation of Elasticity, Pliability and Colour of Thin Split-Thickness Skin Grafts on Deep Dermal and Muscular Wound Bed Within the Course of 1 Year.

International wound journal·2026
Same author

Social Media for Marketing Surgical Practice: Instagram Use by German Plastic Surgeons.

Plastic and reconstructive surgery. Global open·2026
Same author

Does Exsanguination Enhance Skin Microcirculation in Remote Ischemic Conditioning Using a Tourniquet? A Randomized Controlled Trial on 50 Healthy Subjects.

Archives of plastic surgery·2026

This study shows that TRAIL and Tarolidine (TRD) effectively induce apoptosis and reduce proliferation in human fibrosarcoma cells. The combination therapy demonstrated enhanced efficacy, highlighting the NFKB pathway

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Disseminated soft tissue sarcoma presents a significant therapeutic challenge due to a lack of effective chemotherapeutics.
  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and Tarolidine (TRD) possess apoptogenic properties.
  • Human fibrosarcoma (HT1080) cells were utilized to investigate the efficacy of TRAIL and TRD.

Purpose of the Study:

  • To evaluate the apoptogenic and anti-proliferative effects of TRAIL and TRD on human fibrosarcoma cells.
  • To investigate the molecular mechanisms underlying the action of TRAIL and TRD, including gene expression and signaling pathway modulation.
  • To assess the synergistic effects of combining TRAIL and TRD in fibrosarcoma treatment.

Main Methods:

  • Cell viability, apoptosis, and necrosis were assessed using TUNEL assays and flow cytometry (Propidium Iodide/Annexin V staining).

Related Experiment Videos

Last Updated: Jun 27, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

  • Gene expression profiling was performed using RNA-microarray, with validation of key genes via RT-PCR.
  • Protein level alterations were analyzed by Western Blot, and NFKB activity was measured using ELISA. Proliferation assays (BrdU) were also conducted.

    • Main Results:

    • Both TRAIL and TRD significantly induced apoptosis and decreased proliferation in HT1080 cells.
    • Treatment with TRAIL and TRD altered the expression of multiple genes involved in apoptotic pathways.
    • The combination of TRD and TRAIL demonstrated a significant increase in apoptosis compared to single-agent treatments, with notable changes in gene expression profiles.
    • NFKB activity was differentially regulated by TRD and the combination therapy compared to TRAIL alone.

    Conclusions:

    • TRAIL and TRD are effective agents for inducing apoptosis and reducing proliferation in human fibrosarcoma.
    • The study implicates the NFKB pathway as a critical regulator in TRAIL/TRD-mediated apoptosis.
    • Combined TRD and TRAIL therapy shows promise for treating human fibrosarcoma by enhancing apoptosis and modulating key molecular pathways.