Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Zebra chorionic gonadotropin: partial purification and characterization.

J R McFarlane1, N M Czekala, H Papkoff

  • 1Hormone Research Institute, University of California, San Francisco 94143.

Biology of Reproduction
|May 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urinary immunoreactive estrogen and pregnanediol-3-glucuronide during the normal menstrual cycle of the female lowland gorilla (Gorilla gorilla).

American journal of primatology·2020
Same author

Direct measurements of urinary estrone conjugates during the normal menstrual cycle of the gorilla (Gorilla gorilla).

American journal of primatology·2020
Same author

Diurnal urinary corticoid excretion in the human and gorilla.

American journal of primatology·2020
Same author

Monitoring hormones in urine and feces of captive bonobos (Pan paniscus).

Primates; journal of primatology·2018
Same author

Fecal corticosterone refects serum corticosterone in Florida Sandhill Cranes.

Veterinary anaesthesia and analgesia·2017
Same author

Effect of exercise training on liver function in adults who are overweight or exhibit fatty liver disease: a systematic review and meta-analysis.

British journal of sports medicine·2016
Same journal

Reduced Circulating Polyamines in Polyendocrine Metabolic Ovarian Syndrome (PMOS) Patients and the Impact of Putrescine on Ovarian Function and Fertility in a Murine PMOS Model.

Biology of reproduction·2026
Same journal

DEVELOPMENTAL PROGRAMMING IN DOGS.

Biology of reproduction·2026
Same journal

Meiosis-Specific Cohesin in Mammalian Germ Cells.

Biology of reproduction·2026
Same journal

The Research of Lactylation in the Field of Reproduction.

Biology of reproduction·2026
Same journal

Mitochondria as Integrative Regulators of Ferroptosis in the Female Reproductive System.

Biology of reproduction·2026
Same journal

Retraction and replacement of: Navigation of Nanos germ cell specification factor to germ granules-posttranscriptional regulation hubs-across species.

Biology of reproduction·2026
See all related articles

Zebra chorionic gonadotropin (zCG) was isolated and found to be bioactive as a luteinizing hormone (LH). This glycoprotein is immunologically similar to other equine and donkey gonadotropins but lacks follicle-stimulating hormone (FSH) activity.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Comparative Physiology

Background:

  • Chorionic gonadotropin (CG) plays a crucial role in maintaining pregnancy across various species.
  • Understanding the specific properties of CG in different equids, like zebras, provides insights into reproductive endocrinology.

Purpose of the Study:

  • To isolate and characterize zebra chorionic gonadotropin (zCG).
  • To assess the biological activity and receptor binding properties of zCG.
  • To compare zCG with other known gonadotropins, including equine (eCG) and donkey (dCG).

Main Methods:

  • Radioimmunoassay (RIA) to measure CG levels in pregnant zebra serum.
  • Glycoprotein fractionation to purify zCG.
  • High-Performance Liquid Chromatography (HPLC) for purity assessment.

Related Experiment Videos

  • Radioimmunoassays (RIAs), radioreceptor assays (RRAs), and Leydig cell assays to determine bioactivity and receptor interactions.

    • Main Results:

    • Zebra serum contains chorionic gonadotropin (zCG) with levels comparable to mares.
    • Purified zCG is a glycoprotein with molecular size smaller than eCG and similar to equine LH.
    • zCG demonstrated bioactivity as a luteinizing hormone (LH) and competed for LH receptors in RRAs.
    • Immunologically, zCG is similar to eCG, equine LH (eLH), dCG, and zebra LH (zLH).
    • Unlike eCG and eLH, zCG showed minimal to no follicle-stimulating hormone (FSH) activity.

    Conclusions:

    • Zebra chorionic gonadotropin (zCG) is a bioactive LH, distinct from eCG/eLH due to its lack of significant FSH activity.
    • zCG shares similarities with dCG, dLH, and zLH in its hormonal profile.
    • These findings contribute to the comparative understanding of gonadotropin function in equids.