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Related Experiment Video

Updated: Jun 27, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
05:30

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

Making patch-pipettes and sharp electrodes with a programmable puller.

Austin L Brown1, Brandon E Johnson, Miriam B Goodman

  • 1Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA. dokbrown@gmail.com

Journal of Visualized Experiments : Jove
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

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This video demonstrates the fabrication of essential electrophysiology tools: glass microelectrodes for patch-clamp and sharp electrodes for intracellular recordings. Learn how programmable pullers create these vital research instruments using heat and controlled pulling methods.

Area of Science:

  • Electrophysiology
  • Neuroscience
  • Biophysics

Background:

  • Glass microelectrodes are fundamental tools in electrophysiology.
  • Their fabrication is crucial for various recording techniques, including patch-clamp and intracellular recording.
  • Programmable pullers are the standard instruments for creating these microelectrodes.

Purpose of the Study:

  • To provide a practical guide on fabricating glass microelectrodes for electrophysiology.
  • To illustrate the distinct methods for creating patch-clamp pipettes and sharp intracellular electrodes.
  • To showcase the use of pre-established programs on programmable pullers for consistent results.

Main Methods:

  • Utilizing programmable pullers with either filament or laser heating.

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Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Pressure-polishing Pipettes for Improved Patch-clamp Recording

Published on: October 22, 2008

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
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Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

Published on: September 27, 2012

Related Experiment Videos

Last Updated: Jun 27, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
05:30

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

Pressure-polishing Pipettes for Improved Patch-clamp Recording
05:12

Pressure-polishing Pipettes for Improved Patch-clamp Recording

Published on: October 22, 2008

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
10:31

Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness

Published on: September 27, 2012

  • Employing gravity, motor, or combined forces for glass capillary drawing.
  • Differentiating fabrication parameters for patch-clamp (heat, gravity) and intracellular (heat, gravity, motor) electrodes.
  • Selecting appropriate glass capillary diameters (e.g., <1.2 mm for patch-clamp, 1.0 mm OD for intracellular).

    • Main Results:

    • Successful fabrication of both patch-clamp and intracellular recording pipettes.
    • Demonstration of how puller programming varies for different electrode types.
    • Highlighting the similarity between intracellular pipette fabrication and injection needle creation.

    Conclusions:

    • Programmable pullers offer a reliable method for producing high-quality electrophysiology microelectrodes.
    • Understanding specific puller programs is key to fabricating electrodes for distinct applications.
    • This video serves as a valuable resource for researchers needing to create their own recording pipettes.