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Updated: Jun 27, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

Neuronal nuclei isolation from human postmortem brain tissue.

Anouch Matevossian1, Schahram Akbarian

  • 1Psychiatry, Brudnick Neuropsychiatric Research Institute, University of Massachusetts Medical School, MA, USA. Anouch.Matevossian@umassmed.edu

Journal of Visualized Experiments : Jove
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

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Researchers developed a new method to isolate and purify neuronal nuclei from frozen human brain tissue. This technique enables single-cell analysis of neuronal chromatin and DNA across the lifespan and in disease.

Area of Science:

  • Neuroscience
  • Epigenetics
  • Molecular Biology

Background:

  • Neuronal nuclei are maintained throughout life, but their chromatin and organization changes during development, aging, and disease are poorly understood.
  • Current DNA and chromatin assays lack single-cell resolution, limited by the cellular heterogeneity of brain tissue.
  • In vitro cell cultures are insufficient for studying long-term epigenetic changes due to limited cell survival.

Purpose of the Study:

  • To develop a protocol for isolating pure neuronal nuclei from postmortem human brain tissue.
  • To enable single-cell resolution studies of neuronal epigenetics across the lifespan and in disease states.
  • To overcome the limitations of cellular heterogeneity in brain tissue analysis.

Main Methods:

  • Extraction of nuclei from frozen, unfixed human postmortem brain tissue using hypotonic lysis buffer.

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Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Published on: April 16, 2021

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Last Updated: Jun 27, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
08:18

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Published on: April 16, 2021

  • Ultracentrifugation and immunotagging of nuclei with anti-NeuN antibody.
  • Separation and collection of labeled neuronal nuclei using fluorescence-activated sorting (FACS).

    • Main Results:

    • A protocol for efficient extraction and purification of neuronal nuclei from frozen human brain tissue was established.
    • The method allows for the isolation of neuronal nuclei, separating them from other cell types.
    • The purified nuclei are suitable for various downstream epigenetic analyses.

    Conclusions:

    • This novel protocol provides a method to study neuronal epigenetics at single-cell resolution using postmortem brain tissue.
    • The technique is applicable to diverse brain regions and species, facilitating lifespan and disease-related epigenetic research.
    • The method supports chromatin immunoprecipitation, DNA methylation, and other assays for comprehensive epigenetic profiling.