Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Survivable Stereotaxic Surgery in Rodents
00:08

Survivable Stereotaxic Surgery in Rodents

Published on: October 6, 2008

Survivable stereotaxic surgery in rodents.

Brenda M Geiger1, Lauren E Frank, Angela D Caldera-Siu

  • 1Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, USA. Brenda.Geiger@tufts.edu

Journal of Visualized Experiments : Jove
|December 17, 2008
PubMed
Summary

This study details measuring brain neurotransmitters in awake rats using microdialysis and HPLC-EC. This method reveals how drugs and stimuli affect dopamine levels in specific brain regions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Steroid Hormone Changes after Short-Term High-Dose Exposure to Cannabidiol in Healthy Subjects: An Exploratory Study.

Medical cannabis and cannabinoids·2026
Same author

Impact of Asynchronous Training on Student's Confidence and Attitudes Regarding Patients With Opioid Use Disorders.

Substance use & addiction journal·2025
Same author

Deletion of murine astrocytic vesicular nucleotide transporter increases anxiety and depressive-like behavior and attenuates motivation for reward.

Molecular psychiatry·2024
Same author

Pan-cancer analysis of co-occurring mutations in RAD52 and the BRCA1-BRCA2-PALB2 axis in human cancers.

PloS one·2022
Same author

Functional Effects of a Neuromelanin Analogue on Dopaminergic Neurons in 3D Cell Culture.

ACS biomaterials science & engineering·2021
Same author

Impact of Brain Insulin Signaling on Dopamine Function, Food Intake, Reward, and Emotional Behavior.

Current nutrition reports·2019

Area of Science:

  • Neuroscience
  • Pharmacology
  • Analytical Chemistry

Background:

  • Measuring extracellular neurotransmitters in awake animals is crucial for understanding central nervous system (CNS) responses to various challenges.
  • Dopamine projections in the midbrain are key targets for studying responses to drugs like d-amphetamine and natural stimuli such as food.

Purpose of the Study:

  • To demonstrate the surgical implantation of guide cannulas in specific rat brain sites.
  • To illustrate the insertion and implantation of a microdialysis probe.
  • To showcase the use of high-performance liquid chromatography with electrochemical detection (HPLC-EC) for measuring extracellular neurotransmitters and metabolites.

Main Methods:

  • Stereotactic surgery for guide cannula implantation in the rat brain.

More Related Videos

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
12:45

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat

Published on: July 19, 2012

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience
07:01

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience

Published on: January 30, 2012

Related Experiment Videos

Last Updated: Jun 27, 2026

Survivable Stereotaxic Surgery in Rodents
00:08

Survivable Stereotaxic Surgery in Rodents

Published on: October 6, 2008

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
12:45

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat

Published on: July 19, 2012

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience
07:01

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience

Published on: January 30, 2012

  • Microdialysis probe insertion and implantation for targeted analyte collection.
  • High-performance liquid chromatography coupled with electrochemical detection (HPLC-EC) for quantifying oxidizable neurotransmitters and metabolites.

    • Main Results:

    • Successful measurement of extracellular basal neurotransmitter levels in specific brain regions of awake rats.
    • Demonstration of site-specific drug delivery and its effects via microdialysis.
    • Characterization of the technique's excellent anatomical and chemical resolution, with a time resolution of 20-30 minutes.

    Conclusions:

    • This video-based protocol provides a comprehensive guide for in vivo microdialysis and HPLC-EC analysis of neurotransmitters.
    • The technique allows for detailed investigation into the mechanism of action of compounds with high site specificity.
    • Complementary electrophysiology methods can be used to enhance real-time neurotransmission monitoring.