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Quantitative determination of aflatoxin B1 in chick liver
Y Espada1, R Guitart, M Arboix
1Department of Veterinary Medicine, Faculty of Veterinary, Autonomous University of Barcelona, Bellaterra, Spain.
Food Additives and Contaminants
|March 1, 1991
Abstract:
A method for the rapid and quantitative determination of aflatoxin B1 from small quantities of liver, around 1-2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC-fluorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin B1 from chick liver was achieved and detection at levels of 0.2-1 ppb was proved feasible. The aflatoxin B1 concentration in chick liver after oral administration is also shown.