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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 27, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Single-photon-counting detector for increased sensitivity in two-photon laser scanning microscopy.

Richard K P Benninger1, William J Ashby, Elisabeth A Ring

  • 1Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.

Optics Letters
|December 17, 2008
PubMed
Summary

We introduced a photon-counting detector to improve sensitivity in fluorescence laser scanning microscopy. This single-photon-counting detector offers better performance at low signal levels compared to traditional methods.

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Video-rate Scanning Confocal Microscopy and Microendoscopy
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Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Related Experiment Videos

Last Updated: Jun 27, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

Area of Science:

  • Microscopy
  • Photon Detection
  • Biophysics

Background:

  • Conventional laser scanning microscopy (LSM) uses photomultiplier tubes with analog current integration.
  • Analog detectors introduce noise during current measurement, limiting sensitivity at low signal levels.

Purpose of the Study:

  • To present the use and characterization of a photon-counting detector for enhanced sensitivity in fluorescence LSM.
  • To improve imaging of low fluorescence intensities.

Main Methods:

  • Implementation of a fast single-photon-counting (SPC) detector on a two-photon laser scanning microscope.
  • Characterization of the SPC detector's performance at low photon flux.

Main Results:

  • The SPC detector demonstrates shot-noise-limited performance at low photon flux.
  • Increased detection sensitivity compared to analog current integration methods.

Conclusions:

  • Photon-counting detectors significantly enhance sensitivity in fluorescence LSM for low signal levels.
  • SPC detectors provide a superior alternative to conventional analog detectors for sensitive imaging applications.