Prions in milk from ewes incubating natural scrapie
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View abstract on PubMed
Summary
This summary is machine-generated.Prion infectivity was detected in milk and colostrum from sheep with scrapie, months before symptoms appear. This finding highlights potential risks for animal and human health from dairy products derived from affected ruminants.
Area Of Science
- Veterinary Medicine
- Neuroscience
- Food Safety
Background
- Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative diseases.
- Prion infectivity in milk was previously unreported, allowing unrestricted entry of dairy products into food chains.
- Recent studies indicated prions in mammary secretions of scrapie-affected ewes.
Purpose Of The Study
- To detect prion infectivity in milk and colostrum from sheep incubating natural scrapie.
- To determine the presence and distribution of abnormal prion protein (PrPSc) in mammary glands.
- To assess the potential role of milk and colostrum in TSE transmission.
Main Methods
- Bioassays using Tg 338 mouse model to quantify prion infectivity.
- Immunohistochemistry and PET blot to detect abnormal prion protein (PrPSc).
- Fractionation of milk and colostrum to isolate infectivity.
Main Results
- Consistent prion infectivity detected in milk and colostrum from sheep incubating scrapie, pre-clinically.
- Abnormal PrPSc found in lacteal ducts and mammary acini, particularly in ewes with Maedi lentivirus-induced lymphoid follicles.
- Prion infectivity present in milk and colostrum from sheep with and without mammary lesions.
- Infectivity recovered in cellular, cream, and casein-whey fractions.
Conclusions
- Milk and colostrum from TSE-incubating small ruminants can contribute to animal TSE transmission.
- This poses a potential risk to human health through consumption of dairy products.
- Further research is needed to fully understand the implications for food safety.
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