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Updated: Jun 27, 2026
GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
Published on: July 17, 2011
Modulation of network oscillatory activity and GABAergic synaptic transmission by CB1 cannabinoid receptors in the
Nicola H Morgan1, Ian M Stanford, Gavin L Woodhall
1School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK.
Abstract:
Cannabinoids modulate inhibitory GABAergic neurotransmission in many brain regions. Within the temporal lobe, cannabinoid receptors are highly expressed, and are located presynaptically at inhibitory terminals. Here, we have explored the role of type-1 cannabinoid receptors (CB1Rs) at the level of inhibitory synaptic currents and field-recorded network oscillations. We report that arachidonylcyclopropylamide (ACPA; 10 microM), an agonist at CB1R, inhibits GABAergic synaptic transmission onto both superficial and deep medial entorhinal (mEC) neurones, but this has little effect on network oscillations in beta/gamma frequency bands. By contrast, the CB1R antagonist/inverse agonist LY320135 (500 nM), increased GABAergic synaptic activity and beta/gamma oscillatory activity in superficial mEC, was suppressed, whilst that in deep mEC was enhanced. These data indicate that cannabinoid-mediated effects on inhibitory synaptic activity may be constitutively active in vitro, and that modulation of CB1R activation using inverse agonists unmasks complex effects of CBR function on network activity.
