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Susceptibility of newly established mouse strain MPS to Mycoplasma pneumoniae infection
Y Sasaki1, A Ogura, K Nakayama
1Department of General Biologics Control, National Institute of Health, Tokyo.
Abstract:
A newly established mouse strain, MPS, which is more sensitive to Mycoplasma pulmonis than ICR, ddY and other mouse strains was examined for its susceptibility to Mycoplasma pneumoniae. In experimental infections with M. pneumoniae, it was observed that M. pneumoniae attached to tracheas of MPS mice, and M. pneumoniae cells were isolated from tracheas and lungs of MPS mice even after four weeks of infection, while no mycoplasmas were isolated from ICR and ddY mice after one week of infection. Specific antibodies against M. pneumoniae were also observed by the Western blotting in the sera of MPS mice infected with M. pneumoniae. Although any lung lesion could not be observed in this work, this newly established mouse strain MPS may be useful for experiments of M. pneumoniae infection, especially for the analysis of strain differences in susceptibility to M. pneumoniae infection.
Insights
A new mouse strain, MPS, shows higher susceptibility to Mycoplasma pneumoniae infection. This MPS mouse model is valuable for studying Mycoplasma pneumoniae infection and strain differences.
Area of Science:
- Microbiology
- Infectious Diseases
- Animal Models
Background:
- Mycoplasma pneumoniae is a significant human respiratory pathogen.
- Understanding host susceptibility is crucial for studying M. pneumoniae infections.
- Existing mouse models may not fully capture M. pneumoniae pathogenesis.
Purpose of the Study:
- To evaluate the susceptibility of a newly established mouse strain (MPS) to Mycoplasma pneumoniae.
- To compare the MPS strain's response to M. pneumoniae with established strains (ICR, ddY).
- To assess the utility of the MPS strain as a model for M. pneumoniae research.
Main Methods:
- Experimental infection of MPS, ICR, and ddY mice with Mycoplasma pneumoniae.
- Isolation of M. pneumoniae from respiratory tissues (trachea, lungs) at various time points post-infection.
- Detection of specific antibodies against M. pneumoniae using Western blotting.
Main Results:
- Mycoplasma pneumoniae successfully infected and persisted in MPS mice for at least four weeks.
- M. pneumoniae was isolated from the tracheas and lungs of MPS mice, but not from ICR and ddY mice after one week.
- Specific antibodies against M. pneumoniae were detected in the sera of infected MPS mice.
Conclusions:
- The MPS mouse strain demonstrates increased susceptibility to Mycoplasma pneumoniae.
- The MPS strain serves as a potentially valuable model for Mycoplasma pneumoniae infection studies.
- This model is particularly useful for analyzing variations in susceptibility to M. pneumoniae infections.