Anionic polymer, poly(methyl vinyl ether-maleic anhydride)-coated beads-based capture of human influenza A and B...

Area of Science:

• Virology
• Materials Science
• Biotechnology

Background:

• Influenza viruses pose a significant public health threat, necessitating efficient detection and recovery methods.
• Current methods for influenza virus capture can be complex and time-consuming.
• Developing novel materials for enhanced viral capture is crucial for diagnostics and research.

Purpose of the Study:

• To develop and validate an anionic magnetic beads-based method for capturing human influenza A and B viruses.
• To utilize poly(methyl vinyl ether-maleic anhydride) [poly(MVE-MA)] to functionalize magnetic beads for improved bioadhesion and charge.
• To assess the infectivity of captured influenza viruses.

Main Methods:

• Anionic magnetic beads were prepared using poly(MVE-MA) for enhanced charge and bioadhesive properties.
• The functionalized magnetic beads were incubated with samples containing influenza viruses (A and B).
• Magnetic separation was employed to isolate virus-bound beads, followed by confirmation using hemagglutinin assay, immunochromatography, Western blotting, egg infection, and cell infection.

Main Results:

• The poly(MVE-MA)-coated magnetic beads successfully captured various strains of influenza A (H1N1, H3N2) and influenza B viruses.
• Confirmation assays demonstrated effective viral adsorption onto the magnetic beads.
• The infectivity of the captured influenza viruses in chicken embryonated eggs and MDCK cells remained comparable to the original samples.

Conclusions:

• The developed anionic magnetic beads method provides an efficient and effective means for capturing infectious human influenza viruses.
• This technique is suitable for recovering viruses from diverse sample types, including nasal aspirates, allantoic fluid, and culture media.
• The method holds potential for broad application in influenza virus diagnostics, research, and therapeutic development.